《植物生理学报》 2014, 50(10): 1608-1612

油桐叶柄高效直接再生体系的建立

林青, 吴玲利, 张琳*, 谭晓风, 龙洪旭, 李泽

中南林业科技大学, 经济林培育与保护省部共建教育部重点实验室, 长沙410004

通信作者：张琳；E-mail: triwoodtim918@126.com；Tel: 0731-85623416

摘　要：

以油桐无菌苗叶柄为外植体, 研究植物生长调节剂对其离体培养及植株再生的影响。结果表明: 叶柄直接诱导不定芽的最佳培养基1/2MS+3.0 mg•L^-1 6-BA+0.05 mg•L^-1 IAA, 诱导率达91.67%; 最佳继代增殖培养基1/2MS+3.0 mg•L^-16-BA+0.05 mg•L^-1 IBA+1.0 mg•L^-1 GA₃, 增殖系数可达4.89; 最佳生根培养基为1/2MS+0.05 mg•L^-1 IBA, 生根率96.18%。炼苗移栽到泥炭土:珍珠岩:黄土=2:1:1的基质中, 成活率达93.55%以上。

关键词：油桐; 叶柄; 直接再生; 植株再生

收稿：2014-07-14   修定：2014-09-25

资助：国家“948”引进项目“木本植物高效遗传转化及外源基因清除技术引进” (2012-4-42)。

Establishment of Highly Efficient and Direct Regeneration System from Petioles of Tung Tree (Vernicia fordii)

LIN Qing, WU Ling-Li, ZHANG Lin*, TAN Xiao-Feng, LONG Hong-Xu, LI Ze

The Key Laboratory of Cultivation and Protection for Non-Wood Forest Trees, Ministry of Education, Central South University of Forestry and Technology, Changsha 410004, China

Corresponding author: ZHANG Lin; E-mail: triwoodtim918@126.com; Tel: 0731-85623416

Abstract:

The effects of plant growth regulators on plant regeneration in vitro were studied using germ-free petioles of tung tree (Vernicia fordii) as explants. The results showed that the fittest medium for shoot differentiation of petioles was 1/2MS+3.0 mg•L^-1 6-BA+0.05 mg•L^-1 IAA, with a 91.67% shoot induction rate; the best medium for shoot multiplication was 1/2MS+3.0 mg•L^-1 6-BA+0.05 mg•L^-1 IBA+1.0 mg•L^-1 GA₃, with the multiplication coefficient reaching 4.89; the best rooting medium was 1/2MS+0.05 mg•L^-1 IBA, with a 96.18% rooting rate. After the culture-bottle seedlings were transplanted into the matrix composed of peat soil, perlite and loess (2:1:1), the survival rate was up to 93.55%.

Key words: Vernicia fordii; petiole; direct regeneration; plant regeneration